Based upon guidance provided by the University System of Georgia, all Georgia Tech sponsored events through June 30, including athletics competitions, are cancelled, postponed or will move to a virtual format.

Monday, March 30 2020
1:00pm - 2:00pm
Krone Engineered Biosystems Building, Room 1005 - Atlanta, GA
For more information:
Add To My Calendar
Microphysiological Systems Seminar Series

"Repeat Cytometry: Improving Measurement of Single Cells in an Optofluidic Device"

Gregory A. Cooksey, Ph.D.
Paul N. Patrone, Ph.D.
Anthony J. Kearsley, Ph.D.
Applied and Computational Mathematics Division 
Microsystems and Nanotechnology Division
National Institute of Standards and Technology (NIST)

Commercial flow cytometers are highly utilized clinical and research instruments that are optimized to make thousands of single-cell measurements per second.  However, inherent instrument variability coupled with changes in operating conditions or procedures can hinder day-to-day and lab-to-lab comparability of data.  Moreover, difficulty developing informed mathematical analyses prevents cytometers from having well-characterized uncertainties, thereby limiting their ability to detect rare events (a task nonetheless suited to their throughput).  To address these issues, we have developed an optofluidic system with multiple measurement regions to improve understanding of object variability and rare event detection. Monolithic construction of integrated waveguides enables replicate measurement regions to be placed around multiple laser interrogation regions along a single microfluidic stream.  We will discuss the physical principles and mathematical analyses that determine fundamental measurement uncertainties of single cells in flow.  In that context, we will discuss design strategies and signals analyses that improve uncertainty of fluorescence intensity, better resolves coincident events, and, overall, provide analytical tools that enable measurement comparability, improve cell counting and rare event detection.